- deny to written informed consent - age less than 50 years - known infection by the human immunodeficiency virus-1 (hiv-1) - severely immunocompromised patients. this exclusion category comprises: - history of congenital immunodeficiency - history of solid organ transplantation - history of bone marrow transplantation - intake of chemotherapy the last two months - intake of radiotherapy the last two months - active hematological or solid tumor malignancy - history of any anti-cytokine therapies - history of oral or intravenous steroids defined as daily doses of 10mg prednisone or equivalent for longer than the last 3 months

- deny to written informed consent - age less than 50 years - known infection by the human immunodeficiency virus-1 (hiv-1) - severely immunocompromised patients. this exclusion category comprises: - history of congenital immunodeficiency - history of solid organ transplantation - history of bone marrow transplantation - intake of chemotherapy the last two months - intake of radiotherapy the last two months - active hematological or solid tumor malignancy - history of any anti-cytokine therapies - history of oral or intravenous steroids defined as daily doses of 10mg prednisone or equivalent for longer than the last 3 months